Fermentation Technology Division focuses on microbial
biodiversity research / IPR leading to the production
of novel bioactive products, biocatalysts, biotransformed
products for human health and agriculture crop yields
increase through microbial fertilizer, biocides and metal
gluconates. This division is well equipped with latest
fermentors from lab scale to pilot scale 4KL and downstream
processing facilities.
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Industrial microbial strains isolation,
identification and characterization for different
novel bioactive products, biocatalysts for health,
agriculture |
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Production of novel bioactives, biocatalysts
for health and agriculture from novel endophytes
and extremophiles |
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Industrial scale production of microbial
enzymes and high value microbial products from wild
type and genetically modified strains |
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Biotransformations using free and
immobilized enzymes for the production of industrially
important molecules |
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Helping Indian industries for scale
up of processes and building confidence in Indian
industry through transferring technology and training
on microbial fermentation processes |
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Isolation, characterization & identification
of microbes, screening for specific microbial enzymes
and secondary metabolites, enantio/ regio-specificity.
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Chemo-profiling of biocatalysts, biotransformations
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Multi-functional fermentation scale
up facility from lab to pilot scale ranging from
5 to 4000L capacity and downstream processing |
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Technology development for agricultural
crop yield increase by biofertilizers, biocontrol
agents and micronutrients. |
Scientific and Technical
| Sr. NO |
Name |
Expertise |
E-mail |
| 1. |
Rajinder Parshad |
Microbial fermentation technology
scale up & down stream processing, Enzymology, Biotransformation,
Enzyme immobilization and bioprocesses development
especially racemic resolution of drugs.. |
rparshad@iiim.res.in
|
| 2. |
K.S. Manhas |
Microbial Fermentation |
ksmanhas@iiim.res.in
|
| 3. |
R.K. Khajuria |
Microbiology, microbial biofertilizer
& biocontrol agent |
rkkhajuria@iiim.res.in
|
| 4. |
Urmila Jamwal |
Microbiology |
ujamwal@iiim.res.in
|
| 5. |
Chandji Raina |
Microbial Fermentation & entophytes
isolation |
craina@iiim.res.in
|
| 6. |
Asha Chaubey |
Microbial Enzymes, Enzymes immobilization,
biotransformation, and biosensors |
achaubey@iiim.res.in
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Supporting staff:
Jasbir Singh
Shimlu Ram
Prithvi Raj
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Isolation of new microorganisms and
entophytes to develop library of microorganisms
for novel bioactives and biocatalysts. |
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Detailed chemo profiling of enzymes/microorganisms,
biotransformations, resolution of chiral drugs/drug
intermediates. |
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Entophytes isolation & characterization
from selected plants for high value products |
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Identification of microbial strains
for various industrially important products and
enzymes and their large scale production |
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Biotransformation for industrial process
development using microbes/ enzymes and immobilized
enzymes for making the bioprocess easy to operate
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Metabolic engineering of microbes
for metabolite production and yields improvement
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Microbial processes for agriculture
crop yield increase through Bio fertilizers, Biocontrol
agents and micronutrients |
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Cost effective and environmental friendly
processes development for Gluconates (Their use
as neutraceuticals, human and veterinary pharmaceuticals,
food, cosmetics and crop yields increase) |
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The department has excellent Micro-processor
controlled fermentation facility from lab scale
to pilot scale (5-4KL capacity) and matching down
stream processing faculties for bacterial, fungal
and yeast based products. |
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State-of–art equipment required for
Microbiology, Enzymology and Bio-process development
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Centrifuges ranging from laboratory
to industrial scale |
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HPLC |
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GC |
(Intramural, Extramural, public-private partnership)
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Microbial culture isolation from
various locations of J&K (Prospecting microbial
biodiversity : Halophiles and extremophiles) :
Microbial strains isolation from various niches
of Leh area from Soil and water samples collected
from high altitude (water samples from hot water
springs of Chumathong- 16000Ft, Pugga, Nobra 15000Ft
and Cold water Lakes of Karzok (Tsomorari 15075
Ft.), Tsoker, Pangong; Soil samples from Karzok
15075Ft, Tanglangla 17500Ft, Changla Pass 17830Ft,
Pangong, Nobra (North and south Pullu) 16000-17000Ft,
Khurdungla18360Ft their cataloguing, database preparation
and characterization according to physiological
and molecular properties. All the strains are cryo
preserved in glycerol and maintained on agar slants
for research work. |
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| Salt lake |
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| Hot water springs |
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| Khurdungla |
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Microbial enzymes production and
their chemoprofiling
The prime mandate of our department is search for
microbial strains for human health / industrially
important enzymes for biotransformations. Microbial
cultures are subjected to screening for specific
microbial enzymes and secondary metabolites (bioactive).
At the moment the main emphasis is given on enantio/
regio-specificity of enzymes. Microbial roles have
also been identified through their detailed chemo-profiling |
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Microbial enzyme immobilization
and biotransformation:
Enzyme immobilization studies being done for making
the enzyme based processes cheaper, cleaner and
easy to operate. Chiral drugs and their intermediates
are well known for application in pharmaceutical
industry, therefore, collaborative work with bioorganic
chemistry being is done for development of novel
resolution processes of important drugs or their
intermediates through free and immobilized enzymes.
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Scale up of fermentation process
for stereospecific enzymes production
Large scale production of enzymes such as glucose
oxidase, glucose dehydrogenases, lipases, esterases,
oxidoreductase, glycosidases is being done in the
department. Scale-up processes of a few of these
enzymes have been demonstrated to industries. |
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Microbial fermentation process
for the production of DHA (docosahaxaenoic acid)
Fermentation technology for production of DHA (cis-4,
7,10,13,16,19-Docosahexaenoic Acid) is being developed
using marine isolated culture. Efforts are also
being made to achieve maximum DHA production by
pathway engineering of microorganisms.
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Microbial Biofertilizer and biopesticides
fermentation processes
Our division has developed microbial strains of
Rhizobium, Azatobacter, Azospirillum developed at
IIIM Jammu and further cultivated under certain
specifically defined physico-chemical conditions
in 500L fermentor at 28 ? 1oC for a maximum period
of 24h for nitrogen fixation for crop yields increase.
Microbial biofertilizer and biopesticides fermentation
processes has also been transferred to various industries
at 500l scale.
Similarly biocontrol agents has also been produced
both by submerged and by solid state fermentation
(SSF) transferred to various Indian industries.
A strain of Trichoderma viride has been developed
which profusely sporulated in submerged fermentation
conditions under certain specifically defined physico-chemical
conditions at 50L fermentor. The process has been
scaled up to 500L scale. |
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Microbial industrial Process development
of metal gluconates
A fermentation process developed and transferred
to industries for Metal gluconates production at
40KL (Production at 1500TPA).
A single step fermentation process wherein starch
hydrolysate is directly converted to gluconates
of Calcium, Sodium, and Barium etc. has been developed.
The process is highly cost effective, less energy
intensive and environmental friendly. The gluconate
salts are used in neutraceuticals, human and veterinary
pharmaceuticals, food and cosmetic industry. The
technology has been transferred to M/S Prathistha
Industries Ltd., Hyderabad and has been running
since May, 2004. In recognition of this achievement
the Institute was awarded
| a) |
CSIR Technology Award 2001, |
| b) |
Vasvik Award 2002 and |
| c) |
64 th All India Industrial
Award for best invention at Hyderabad 2004.
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Metal ion gluconates use in agriculture:
The products like bio potash and bio zinc produced
by this process are also being marketed by the company
through out the country as an outcome of our technology
for crop yield increase and now many more industries
are approaching us for this technology package.
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| NWP006: Bio prospecting microbial
strains from North Western Himalayas (CSIR Networking
2007-2012) |
| RSP0028 : Rural development
programme including microbial Biofertilizer Biopesticides
and Mosquito eradication (CSIR Networking 2007-2012) |
| Industrial Projects: |
| 1. |
Fermentation process for the production
of metal gluconates & Technical guidance for up
setting a plant for the production of metal gluconates,
enzyme preparation and biofertilizer production
M/s Subash Fertilizer, Alana Aurangabad, India
( Sept.2008- 2011) |
| 2. |
Fermentation process for the production
of metal gluconates and bacterial malarial larvicides
M/s Sribiotech Hyderabad. ( Sept.2008- 2011) |
| 3. |
Fermention process for production
of DHA (docosahaexaenic acid) M/s Avesthagen
Bangalore (2007- 2010) |
| 4. |
Microbial Biocides and protein hydrolysate
production process M/s Glow Biotech Ltd Aligarh
(May 2009- 2012) |
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