Analytical Chemistry division of IIIM Jammu is actively
involved in Pharmacokinetic/ADME studies of structurally
and therapeutically different drugs, and their validation;
development of validated HPLC, HPLC-MS and HPLC-MS/MS
methodologies for the standardization of plant extracts,
active fractions & sub fractions of herbal and poly herbal
formulations; separation, identification and purification
of the natural products with the help of Liquid Chromatography-Mass
Spectrometer (LC-MS).
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To develop HPLC, HPLC-MS methodologies
for the ADME studies of structurally and therapeutically
different drugs in different matrices such as bio-fluids
such as blood, plasma , serum & urine and edible
foods such as honey , milk and meat. |
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To develop HPLC-MS and HPLC methods
for the standardization of plant extracts, fractions
and sub-fractions, for drawing the chemical fingerprints
of the plant extracts. |
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To carry out the trace analysis of
antibiotics in honey by MRM in LC-MS. The analysis
is done to ensure the purity of the products for
export to EU and US through Export Inspection Council
of India. |
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Isolation and characterization of
the natural products from the medicinal and aromatic
plants used in the herbal preparations by using
techniques like thin Layer Chromatography (TLC),
Column Chromatography (CC) and modern techniques
like Medium Pressure Liquid Chromatography (MPLC)
coupled with the use of spectroscopic techniques
like 1H NMR, 13C NMR, LC-MS, IR and UV for structure
elucidation. Besides the already known compounds
a galaxy of unknown compounds having diversified
structures have been isolated from the plants. Plants
bearing edible colors have also been screened for
the color bearing molecules. |
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A large no. of plants exhibiting various
pharmacological activities such as Antidepressant,
Immunomodulatory, Bioavailability enhancing activity
have been screened by using LC-MS. |
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Sepbox, a two dimensional chromatography
system is used for the separation of the constituents
from the huge mixtures like plant extracts and combinatorial
products. The fraction collected from the system
can be directly transferred to MTPS (Medium through
put screens) or HTPS (High through put screens)
for the biological assays. |
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The plants and the pure molecules
analysed by HPLC, HPLC-MS and HPLC-MS/MS are as
under:
| 1. |
Hypericum perforatum |
2. |
Echinacea purpurea, |
| 3. |
Withania somnifera |
4. |
Argyrolobium roseum |
| 5. |
Vitex negundo |
6. |
Colebrookeia oppositifolia
|
| 7. |
Boswellia serratta |
8. |
Thevetia peruviana |
| 9. |
Embelica officinalis |
10. |
Zingiber officinalis |
| 11. |
Dioscorea deltoida |
12. |
Valerian officinalis, |
| 13. |
Podophyllum emodi |
14. |
Crocus sativus, |
| 15. |
Swertia chirayita |
16. |
Tinospora cordifolia |
| 17. |
Carum carvi |
18. |
Cuminum cyminum |
| 19. |
Cesrtum diurnum |
20. |
Picrorrhiza kurroa |
| 21. |
Bacopa monneiri |
22. |
Centella asiatica |
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Pharmacokinetic studies of structurally
and therapeutically different drugs were carried
out in biological fluids in order to find out their
therapeutic efficacy and bioavailability with and
without a naturally occurring bioavailability enhancer.
The most important of them are the Rifampicin, Pyrazinamide
and isoniazid. These drugs have been studied both
as such and in combination with piperine which was
used as a bio-enhancer. |
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LC-MSn , Ion-trap |
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LC-Q-TOF |
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MALDI-TOF |
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Analytical HPLCs |
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Sepbox |
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Preparative HPLC |
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We are engaged in development of analytical
methodologies for the analysis of drugs in different
matrices such as plant extracts monoherbal and polyherbal
formulations, biofluids such as blood, serum and
urine. Under the golden triangle project (GTP) we
are standardizing number of monoherbal and polyherbal
formulations. The methodologies for the HPLC and
HPLC-MS analysis of natural molecules such as piperine,
Gallic acids and withanolides were developed as
these compounds form the major constituents of these
herbal formulations. |
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LC-MS is also being used to characterize
molecules on the basis of MS/MS studies. We are
also developing the LC-MS methods for analysis of
different drugs for pharmacokinetic studies. The
plant extracts are being standardized on the basis
of biomarker compounds. The different accessions
of a plant species are being cultivated as well
as collected from different regions of the country
and these wild as well as cultivated collections
are being studied for the amounts of the marker
compounds present in these accessions. This type
of studies provides information about the quality
of the plant material which exist at a certain location.
The components present in the endophytic fungi and
fermentation broths are also being determined and
quantified with the help of LC-MS and LC-MS/MS.
The metabolites of the drugs are being studied in
different biofluids with the help of HPLC and HPLC-MS |
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Natural/ Synthetic compounds (INF0007)
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Establishment of National Referral
Lab (GAP0195) |
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Development / commercialization of
biomolecules from plant sources (COR0023) |
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Development of natural products as
Bioenhancers for oral care, Colgate Palmolive Sponsored
project (Project No. SSP-0406) |
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Natural additives for baby care wipes
formulations Proctor and Gamble sponsored project
(SSP0404) |
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Validation of traditional ayurvedic
drugs Golden triangle project (GAP-401) |
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